CRISPR-Chip Innovation Featured in The Scientist

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Searching a sample of DNA for a particular sequence—be it a mutation, a researcher-inserted transgene, or evidence of an infecting organism—is a common practice in many molecular biology and diagnostic laboratories around the world. Often, such searches take the form of target amplification, which involves using sequence-specific oligonucleotide primers and the action of a DNA polymerase to pull out the sequence of interest. But amplification not only adds a step to the search process—requiring optimization, reagents, and time—it can also introduce errors such as amplification bias.

Aran Lab